ESPECTROSCOPIA UV VISIBLE PDF

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Scientific Supervisor: Ricardo Molina. The activities developed by the Infrared and ultraviolet-visible Spectroscopy Service have focused, in recent years, on the qualitative and quantitative characterization of materials and chemical compounds.

It is worth highlighting the use of infrared spectroscopy for the characterization of polymeric nanoparticles, coatings in textile materials, advanced polymeric films, liposomes or chemicals in solution and degradation kinetics of chemical or radical compounds. Ricardo Molina Scientific Supervisor ricardo. In this way, transmission measurements of chemical and pharmaceutical products can be performed in KBr pellets or over NaCl crystals. UV-Visible Cary spectrophotometer nm in order to perform characterization, quantitative analysis and reactions kinetics or chemical compounds.

SpectraMAX M5 multi-mode microplate reader nm — nm. For life sciences research, the system is well suited for ELISA, nucleic acid, protein quantification, enzymatic, cell viability and proliferation assays. This site uses cookies. By continuing to browse the site, you are agreeing to our use of cookies. We may request cookies to be set on your device. We use cookies to let us know when you visit our websites, how you interact with us, to enrich your user experience, and to customize your relationship with our website.

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Changes will take effect once you reload the page. Presentation Services. Scientific Supervisor: Ricardo Molina The activities developed by the Infrared and ultraviolet-visible Spectroscopy Service have focused, in recent years, on the qualitative and quantitative characterization of materials and chemical compounds. The Infrared and ultraviolet-visible spectroscopy service is equipped with:. OK Learn more. Cookie and Privacy Settings.

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UV-Vis Spectral Databases

This means it uses light in the visible and adjacent ranges. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum , atoms and molecules undergo electronic transitions. Absorption spectroscopy is complementary to fluorescence spectroscopy , in that fluorescence deals with transitions from the excited state to the ground state , while absorption measures transitions from the ground state to the excited state. Molecules containing bonding and non-bonding electrons n-electrons can absorb energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied. The Beer—Lambert law states that the absorbance of a solution is directly proportional to the concentration of the absorbing species in the solution and the path length.

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